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350 internal-control plasmid encoding renilla luciferase (phrl-tk  (Promega)

 
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    Structured Review

    Promega 350 internal-control plasmid encoding renilla luciferase (phrl-tk
    350 Internal Control Plasmid Encoding Renilla Luciferase (Phrl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phrl-tk+internal+control+plasmid+encoding+renilla+luciferase/350+internal+control+plasmid+encoding+renilla+luciferase++phrl+tk/pm25814121-151-6-13
    Average 90 stars, based on 1 article reviews
    350 internal-control plasmid encoding renilla luciferase (phrl-tk - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: PPAR? inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism
    Article Snippet: Plasmid DNA samples were 800 ng/well of a full-length MUC1 cDNA ( 38 ), or the pcDNA3.1 empty vector control, or a MUC1 promoter-firefly luciferase reporter plasmid (MUC1-pGL2b; Ref. 30 ), or the empty pGL2b vector control, plus 10 ng/well of phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI).

    Article Title: Suppression of IL-8 production in gastric epithelial cells by MUC1 mucin and peroxisome proliferator-associated receptor-?
    Article Snippet: AGS cells in 24-well plates were incubated for 24 h, the medium was replaced with DMEM/F-12 containing 0.1% FBS, and the cells were incubated for an additional 24 h. The cells were cotransfected with 80 ng/well of a Muc1 promoter-firefly luciferase reporter plasmid (Muc1-pGL2b), an IL-8 promoter-lucifease reporter plasmid (IL-8-pGL2b), or with the empty pGL2 vector, plus 10 ng/well of the phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI) using Lipofectamine (Invitrogen) as we described ( 18 , 28 ).

    Article Title: TNF-alpha induces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.
    Article Snippet: Koga T, Kuwahara I, Lillehoj EP, Lu W, Miyata T, Isohama Y, Kim KC.. TNFinduces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.. Am J Physiol Lung Cell Mol Physiol 293: L693–L701, 2007.

    Control:

    Article Title: PPAR? inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism
    Article Snippet: Plasmid DNA samples were 800 ng/well of a full-length MUC1 cDNA ( 38 ), or the pcDNA3.1 empty vector control, or a MUC1 promoter-firefly luciferase reporter plasmid (MUC1-pGL2b; Ref. 30 ), or the empty pGL2b vector control, plus 10 ng/well of phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI).

    Article Title: Suppression of IL-8 production in gastric epithelial cells by MUC1 mucin and peroxisome proliferator-associated receptor-?
    Article Snippet: AGS cells in 24-well plates were incubated for 24 h, the medium was replaced with DMEM/F-12 containing 0.1% FBS, and the cells were incubated for an additional 24 h. The cells were cotransfected with 80 ng/well of a Muc1 promoter-firefly luciferase reporter plasmid (Muc1-pGL2b), an IL-8 promoter-lucifease reporter plasmid (IL-8-pGL2b), or with the empty pGL2 vector, plus 10 ng/well of the phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI) using Lipofectamine (Invitrogen) as we described ( 18 , 28 ).

    Article Title: TNF-alpha induces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.
    Article Snippet: Koga T, Kuwahara I, Lillehoj EP, Lu W, Miyata T, Isohama Y, Kim KC.. TNFinduces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.. Am J Physiol Lung Cell Mol Physiol 293: L693–L701, 2007.

    Luciferase:

    Article Title: PPAR? inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism
    Article Snippet: Plasmid DNA samples were 800 ng/well of a full-length MUC1 cDNA ( 38 ), or the pcDNA3.1 empty vector control, or a MUC1 promoter-firefly luciferase reporter plasmid (MUC1-pGL2b; Ref. 30 ), or the empty pGL2b vector control, plus 10 ng/well of phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI).

    Article Title: Suppression of IL-8 production in gastric epithelial cells by MUC1 mucin and peroxisome proliferator-associated receptor-?
    Article Snippet: AGS cells in 24-well plates were incubated for 24 h, the medium was replaced with DMEM/F-12 containing 0.1% FBS, and the cells were incubated for an additional 24 h. The cells were cotransfected with 80 ng/well of a Muc1 promoter-firefly luciferase reporter plasmid (Muc1-pGL2b), an IL-8 promoter-lucifease reporter plasmid (IL-8-pGL2b), or with the empty pGL2 vector, plus 10 ng/well of the phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI) using Lipofectamine (Invitrogen) as we described ( 18 , 28 ).

    Article Title: TNF-alpha induces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.
    Article Snippet: Koga T, Kuwahara I, Lillehoj EP, Lu W, Miyata T, Isohama Y, Kim KC.. TNFinduces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.. Am J Physiol Lung Cell Mol Physiol 293: L693–L701, 2007.

    Incubation:

    Article Title: PPAR? inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism
    Article Snippet: Plasmid DNA samples were 800 ng/well of a full-length MUC1 cDNA ( 38 ), or the pcDNA3.1 empty vector control, or a MUC1 promoter-firefly luciferase reporter plasmid (MUC1-pGL2b; Ref. 30 ), or the empty pGL2b vector control, plus 10 ng/well of phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI).

    Article Title: Suppression of IL-8 production in gastric epithelial cells by MUC1 mucin and peroxisome proliferator-associated receptor-?
    Article Snippet: AGS cells in 24-well plates were incubated for 24 h, the medium was replaced with DMEM/F-12 containing 0.1% FBS, and the cells were incubated for an additional 24 h. The cells were cotransfected with 80 ng/well of a Muc1 promoter-firefly luciferase reporter plasmid (Muc1-pGL2b), an IL-8 promoter-lucifease reporter plasmid (IL-8-pGL2b), or with the empty pGL2 vector, plus 10 ng/well of the phRL-TK internal control plasmid encoding Renilla luciferase (Promega, Madison, WI) using Lipofectamine (Invitrogen) as we described ( 18 , 28 ).

    Article Title: TNF-alpha induces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.
    Article Snippet: Koga T, Kuwahara I, Lillehoj EP, Lu W, Miyata T, Isohama Y, Kim KC.. TNFinduces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication.. Am J Physiol Lung Cell Mol Physiol 293: L693–L701, 2007.



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